On the contrary, all the twelve getPCR strategies with different watching primers could accurately determine the indel frequencies Fig. RNA-guided human genome engineering via Cas9. Mean expression score and mean fusion score were calculated by taking the average of the expression scores and fusion scores of all mutants, respectively, at that position. Protein engineering strategies for rational immunogen design. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind.
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