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All Images were taken after 3 days on sporulation media. We found that none of the genes from syntenic loci group together in a well-supported clade that excludes genes from other loci Figure 5 , Figure 5—source data 1 , Figure 5—figure supplement 1. We aimed to identify the mechanism of WT KIT loss and to determine whether other genes are involved or affected. To test this possibility, we compared the amount of Wtf4 poison signal in spores produced by diploids heterozygous for both p antidote -wtf4 poison -GFP allele at ade6 and wtf4 antidote -mCherry at ura4 to similar control diploids in which wtf4 poison -GFP was expressed from its endogenous promoter Fig 5A and 5B. Nat Commun.

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